The Extraction of Plasma 3-hydroxy-17-oxo Steroid Sulphates and the Measurement of the Constituent Dehydroepiandrosterone Sulphate and Androsterone Sulphate.
نویسندگان
چکیده
1. A simple method for the extraction of 17-oxo steroid sulphates of plasma is described; glucosiduronates and orthophosphates are extracted, but to a smaller extent. 2. Four methods of analyses of the extracts are given and are relatively simple. Three of these are specific for steroid sulphates and two measure the sulphate conjugates directly. 3. Values for dehydroepiandrosterone sulphate and androsterone sulphate concentrations of normal and pathological plasmas are given.
منابع مشابه
Isolation and characterization of 17 -hydroxy steroid dehydrogenase from human erythrocytes.
1. The 17beta-hydroxy steroid dehydrogenase was solubilized during haemolysis of erythrocytes and was isolated from the membrane-free haemolysate. Membrane preparations isolated in different ways did not contain 17beta-hydroxy steroid dehydrogenase activity. The 17beta-hydroxy steroid dehydrogenase activity in the haemolysate was concentrated by repeated ammonium sulphate precipitation and gel ...
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عنوان ژورنال:
- The Biochemical journal
دوره 95 شماره
صفحات -
تاریخ انتشار 1965